dnaD [2017-12-07 13:27:39]

initiation of chromosome replication, part of the replisome

locus: BSU22350

pI: 7.17

mw: 27.49 kDa

protein length: 232 aa

gene length: 696 bp

function: DNA replication

product: initiation of chromosome replication

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,345,433 → 2,346,131

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|DnaD]-mediated untwisting of the DNA stimulates [protein|C352560155F5B2E951B5059794D7B319C1A3AF2C|Nth] endonuclease activity at AP sites [Pubmed|21954439]
• required for the assembly of the replicative helicase DnaC at the origin of replication [Pubmed|22821970]
• interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA [Pubmed|22821970]

Protein family

• DNA polymerase type-Y family (according to Swiss-Prot)

Structure

• [PDB|2C79] (N-terminal domain)

[SW|Localization]

• Membrane-proximal (Spotty) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|FBA7EAA70F5C9E76463B0C5F85D65D4314FFD40D|asnS]-[gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]-[gene|4B2A124B726393544A47791E5A5681F7B766A588|ypoC]
  description: [Pubmed|22383849]

• genes: [gene|C9FEE07601AD28B2651EB881A1D9B34D5082237C|dnaD]-[gene|C352560155F5B2E951B5059794D7B319C1A3AF2C|nth]
  description: [Pubmed|21954439]

  regulation

  • constitutively expressed [Pubmed|21954439]

Labs working on this gene/protein

• [SW|Alan Grossman], MIT, Cambridge, MA, USA

References

Reviews

• 22797751,28075389

Original publications

• 19707554,15686560,15186423,11585815,20122408,16479537,11222620,19968790,11679082,20587500,21097613,21954439,22821970